bar
Publications

PUBLISHED

Peters J.W., Beratan, D.M., Schut, G.J., Adams, M.W.W. On the nature of organic and inorganic centers that bifurcate electrons, coupling exergonic and endergonic oxidation–reduction reactions. Chem Comm. 2018. [DOI: 10.1039/C8CC01530A]

Mus, F., Alleman, A.B., Pence, N., Peters, J.W. Exploring the alternatives of biological nitrogen fixation. Metallomics. 2018. [DOI: 10.1039/C8MT00038G]

Berry, L., Patterson, A., Pence, N., Peters, J.W., Bothner, B. Hydrogen deuterium exchange mass spectrometry of oxygen sensitive proteins. Bio-protocol. 2018. [DOI: 10.21769/BioProtoc.2769]

Artz, J.H., Mulder, D.W., Poudel, S., Colman, D., Schut, G.J., Williams, S.G., Jones, A.K., Adams, M.W.W., Boyd, E.S., King, P.W., Peters, J.W. Structure-Function of [FeFe]- and [NiFe]-Hydrogenases: An Overview of Diversity, Mechanism, Maturation, and Bifurcation. In book: Microalgal Hydrogen Production: Achievements and Perspectives, Chapter: 2, Publisher: RSC Publishing, Editors: Seibert, M., Torzillo, G., pp. 31 - 66. [DOI: 10.1039/9781849737128-00031]

Poudel, S., Colman, D.R., Fixen, K.R., Ledbetter, R.N., Zheng, Y., Pence, N., Seefeldt, L.C., Peters, J.W., Harwood, C.S.,, Boyd, E.S. Electron transfer to nitrogenase in different genomic and metabolic backgrounds. J Bacteriol. 2018. [DOI: 10.1128/JB.00757-17]

Duan H.D., Lubner, C. E., Tokmina-Lukaszewska, M., Gauss, G.H., Bothner, B., King, P.W., Peters, J.W., Miller, A.F. Distinct flavin properties underlie flavin-based electron bifurcation within a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris Transduction in Nitrogenase. J Biol Chem. 2018. [DOI: 10.1074/jbc.RA117.000707]

Harris, D.F., Lukoyanov, D., Shaw, S., Compton, P., Tokmina-Lukaszewska, M., Bothner, B., Kelleher, N., Dean, D.R., Hoffman, B.M., Seefeldt, L.C. The mechanism of N2 reduction by Fe-Nitrogenase involves reductive elimination of H2. Biochemistry. 2018. 57 (5), 701-710. [DOI: 10.1021/acs.biochem.7b01142]

Zheng, Y., Harris, D.F., Yu, Z., Fu, Y., Poudel, S., Ledbetter, R.N., Fixen, K.R., Yang, Z.-Y., Boyd, E.S., Lidstrom, M.E., Seefeldt, L.C., Harwood, C.S. A pathway for biological methane production using iron-only nitrogenase. Nat Microbiol. 2018, 3 (3), 281-286. [DOI: 10.1038/s41564-017-0091-5]

Berry, L., Poudel, S., Tokmina-Lukaszewska, M., Colman, D.R., Nguyen, D.M.N., Schut, G.J., Adams, M.W.W., Peters, J.W., Boyd, E.S., Bothner, B. H/D exchange mass spectrometry and statistical coupling analysis reveal a role for allostery in a ferredoxin-dependent bifurcating transhydrogenase catalytic cycle. Biochim Biophys Acta. 2018. 1862 (1), 9-17. [DOI: 10.1016/j.bbagen.2017.10.002]

Greene BL, Vansuch GE, Chica BC, Adams MWW, Dyer RB. Applications of Photogating and Time Resolved Spectroscopy to Mechanistic Studies of Hydrogenases. Acc Chem Res. 2017. [DOI: 10.1021/acs.accounts.7b00356]

Greene BL, Schut GJ, Adams MWW, Dyer RB. Pre-steady state kinetics of catalytic intermediates of an [FeFe]-hydrogenase. ACS Catalysis. 2017. [DOI: 10.1021/acscatal.6b03276]

Zhang P, Yuly JL, Lubner CE, Mulder DW, King PW, Peters JW, Beratan DN. Electron Bifurcation: Thermodynamics and Kinetics of Two-Electron Brokering in Biological Redox Chemistry. Acc Chem Res. 2017. [DOI: 10.1021/acs.accounts.7b00327]

Berry L, Poudel S, Tokmina-Lukaszewska M, Colman DR, Nguyen DMN, Schut GJ, Adams MWW, Peters JW, Boyd ES, Bothner B. H/D exchange mass spectrometry and statistical coupling analysis reveal a role for allostery in a ferredoxin-dependent bifurcating transhydrogenase catalytic cycle.. Biochim Biophys Acta. 2017. [DOI: 10.1016/j.bbagen.2017.10.002]

Peters JW, Lubner C. Electron Bifurcation Makes the Puzzle Pieces Fall Energetically into Place in Methanogenic Energy Conservation. Chembiochem. 2017. [DOI: 10.1002/cbic.201700533]

Artz JH, Zadvornyy OA, Mulder DW, King PW, Peters JW. Structural Characterization of Poised States in the Oxygen Sensitive Hydrogenases and Nitrogenases. Methods Enzymol. 2017. [DOI: 10.1016/bs.mie.2017.07.005]

Garcia Costas AM, Poudel S, Miller AF, Schut GJ, Ledbetter RN, Fixen KR, Seefeldt LC, Adams MWW, Harwood CS, Boyd ES, Peters JW. Defining Electron Bifurcation in the Electron Transferring Flavoprotein Family. J Bacteriol. 2017. [DOI: 10.1128/JB.00440-17]

Losey NA, Mus F, Peters JW, Le HM, McInerney MJ. Syntrophomonas wolfei Uses a NADH-Dependent, Ferredoxin-Independent, [FeFe]-Hydrogenase to Reoxidize NADH. Appl Environ Microbiol. 2017. [DOI: 10.1128/AEM.01335-17]

Pence N, Tokmina-Lukaszewska M, Yang ZY, Ledbetter RN, Seefeldt LC, Bothner B, Peters JW. Unraveling the interactions of the physiological reductant flavodoxin with the different conformations of the Fe protein in the nitrogenase cycle. J Biol Chem. 2017. [DOI: 10.1074/jbc.M117.801548]

Therien JB, Artz JH, Poudel S, Hamilton TL, Liu Z, Noone SM, Adams MWW, King PW, Bryant DA, Boyd ES, Peters JW. The Physiological Functions and Structural Determinants of Catalytic Bias in the [FeFe]-Hydrogenases CpI and CpII of Clostridium pasteurianum Strain W5. Front Microbiol. 2017. [DOI: 10.3389/fmicb.2017.01305]

Nguyen, Diep M. N., Schut, Gerrit, Poudel, Saroj, Zadvornyy, Oleg A., Tokmina-Lukaszewska, Monika, Lipscomb, Gina L., Adams, Leslie A., Dimsmore, Jessica, Nixon, Will, Bothner, Brian, Boyd, Eric, Peters, John W., and Adams, Michael W.W. Two Functionally Distinct Bifurcating NADP-Dependent Ferredoxin Oxidoreductases Maintain the Primary Redox Balance of Pyrococcus furiosus. J Biol Chem. 2017. [DOI: 10.1074/jbc.M117.794172].

Ledbetter, Rhesa N., Garcia Costas, Amaya M., Lubner, Carolyn E., Mulder, David W., Tokmina-Lukaszewska, Monika, Artz, Jacob H., Patterson, Angela, Magnuson,Timothy S., Jay, Zackary J. H., Duan, Diessel, Miller, Jacquelyn, Plunkett, Mary H., Hoben, John P., Barney, Brett M., Carlson, Ross P., Miller, Anne-Frances, Bothner, Brian, King, Paul W., Peters, John W. Peters, and Seefeldt, Lance C. The Electron Bifurcating FixABCX Protein Complex from Azotobacter vinelandii: Generation of Low-Potential Reducing Equivalents for Nitrogenase Catalysis. Biochemistry. 2017. [DOI: 10.1021/acs.biochem.7b00389].

Artz, Jacob H., Mulder, David, Ratzloff, Michael, Lubner, Carolyn, Zadvornyy, Oleg, LeVan, Axl, Williams, S., Adams, Michael W.W., Jones, Anne K., King, Paul W., and Peters, John W. The reduction potentials of [FeFe]-hydrogenase accessory iron-sulfur clusters provide insights into the energetics of proton reduction catalysis. J Am Chem Soc. 2017. [DOI: 10.1021/jacs.7b02099].

Hoben, John P., Lubner, Carolyn E., Schut. Gerrit J., Nguyen, Diep M. N., Hempel, Karl W., Adams, Michael W. W., King, Paul W., and Miller, Anne-Frances. Equilibrium and ultrafast kinetic studies manipulating electron transfer: a short-lived flavin semiquinone is not sufficient for electron bifurcation. J Biol Chem. 2017. [DOI: 10.1074/jbc.M117.794214].

Lubner, Carolyn E., Jennings, David P., Mulder, David W., Schut, Gerrit J., Zadvornyy, Oleg A., Hoben, John P., Tokmina-Lukaszewska, Monika, Berry, Luke, Nguyen, Diep M., Lipscomb, Gina L., Bothner, Brian, Jones, Anne K., Miller, Anne-Frances, King, Paul W., Adams, Michael W. W., and Peters, John W. Mechanistic insights into energy conservation by flavin-based electron bifurcation. Nat Chem Biol. 2017. [DOI: 10.1038/nchembio.2348]

Hoben, John P., Wang, Jianing, and Miller, Anne-Frances. Unboiling an Egg: An Introduction to Circular Dichroism and Protein Refoldin. J Chem Educ. 2016. [DOI: 10.1021/acs.jchemed.6b00319]

Danyal, Karamatullah, Shaw, Sudipta, Page, Taylor R., Duval, Simon, Horitani, Masaki, Marts, Amy R., Lukoyanov, Dmitriy, Dean, Dennis R., Raugei, Simone, Hoffman, Brian M., Seefeldt, Lance C., and Antony, Edwin. Negative cooperativity in the nitrogenase Fe protein electron delivery cycle. Proc Natl Acad Sci U S A. 2016. [DOI: 10.1073/pnas.1613089113].

Fixen, Kathryn R., Zheng, Yanning, Harris, Derek F, Shaw, Sudipta, Yang, Zhi-Yong, Dean, Dennis R, Seefeldt, Lance C., and Harwood, Caroline S. Light-driven carbon dioxide reduction to methane by nitrogenase in a photosynthetic bacterium. Proc Natl Acad Sci U S A. 2016. [DOI: 10.1073/pnas.1611043113]

Yang, Zhi-Yong, Ledbetter, Rhesa, Shaw, Sudipta, Pence, Natasha K., Tokmina-Lukaszewska, Monika, Eilers, Brian J., Guo, Qingjuan, Pokhrel, Nilisha, Cash, Valerie L., Dean, Dennis R., Antony, Edwin, Bothner, Brian, Peters, John W., and Seefeldt, Lance C. Evidence that the Pi Release Event is the Rate Limiting Step in the Nitrogenase Catalytic Cycle. Biochemistry. 2016. [10.1021/acs.biochem.6b00421]

Poudel, Saroj, Tokmina-Lukaszewska, Monika, Colman, Daniel R., Refai, Mohammed, Schut, Gerrit J., King, Paul W., Maness, Pin-Ching, Adams, Michael W. W., Peters, John W., Bothner, Brian, and Boyd, Eric S. Unification of [FeFe]-hydrogenases into three structural and functional groups. Biochim Biophys Acta Gen Subj. 2016. [DOI: 10.1016/j.bbagen.2016.05.034]

Brown KA, Harris DF, Wilker MB, Rasmussen A, Khadka N, Hamby H, Keable S, Dukovic G, Peters JW, Seefeldt LC, King PW. Light-driven Dinitrogen reduction catalyzed by a CdS:nitrogenase MoFe protein biohybrid. Science. 2016. [10.1126/science.aaf2091].

Peters, John W., Miller, Anne-Frances, Jones, Anne K., King, Paul W., and Adams, Michael W. W. Electron bifucation. Curr Opin Chem Biol. 2016. [10.1016/j.cbpa.2016.03.007]

Schut, Gerrit J., Lipscomb, Gina L., Nguyen, Diep M. N., Kelly, Robert M., and Adams, Michael W. W. Heterologous production of an energy-conserving carbon monoxide dehydrogenase complex in the hyperthermophile Pyrococcus furiosus. Front Microbiol. 2016. [10.3389/fmicb.2016.00029]

Schut, Gerrit J., Zadvornyy, Oleg A., Wu, Chang-Hao, Peters, John W., Boyd, Eric S., and Adams, Michael W. W. The role of geochemistry and energetics in the evolution of modern respiratory complexes from a proton-reducing ancestor. Biochim Biophys Acta Bioenerg. 2016. [10.1016/j.bbabio.2016.01.010]

Cohen, Aina E., Soltis, S. Michael, Gonzalez, Ana Z., Aguila, Laura, Alonso-Mori, Roberto, Barnes, Christopher O., Baxter, Elizabeth L., Brehmer, Winnie, Brewster, Aaron S., Brunger, Axel T., Calero, Guillermo, Chang, Joseph F., Chollet, Matthieu, Ehrensberger, Paul, Eriksson, Thomas L., Feng, Yiping, Hattne, Johan, Hedman, Britt, Hollenbeck, Michael, Holton, James M., Keable, Stephen, Kobilka, Brian K., Kovaleva, Elena G., Kruse, Andrew C., Lemke, Henrik T., Lin, Guowu, Lyubimov, Artem Y., Manglik, Aashish, Mathews, Irimpan I., McPhillips, Scott E., Nelson, Silke, Peters, John W., Sauter, Nicholas K., Smith, Clyde A., Song, Jinhu, Stevenson, Hilary P., Tsai, Yingssu, Uervirojnangkoorn, Monarin, Vinetsky, Vladimir, Wakatsuki, Soichi, Weis, William I., Zadvornyy, Oleg A., Zeldin, Oliver B., Zhu, Diling, and Hodgson, Keith O. Goniometer-based femtosecond crystallography with X-ray free electon lasers. Proc Natl Acad Sci U S A. 2014. [10.1073/pnas.1418733111]

Peters, John W., Schut, Gerrit J., Boyd, Eric S., Mulder, David W., Shepard, Eric M., Broderick, Joan B., King, Paul W., and Adams, Michael W. W. [FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation. Biochim Biophys Acta Mol Cell Res. 2015. [10.1016/j.bbamcr.2014.11.021]

SUBMITTED/UNDER REVIEW

Fixen, K.R., Chowdury, N.P., Martinez, M., Poudel, P., Boyd, E.S., Harwood, C.S. Electron transfer to nitrogenase in a phototrophic alpha-proteobacterium. Environ Microbiol – Revision Submitted

Yu, H., Wu, C.-H., Schut, G.J., Zhao, G., Peters, J.W., Adams, M.W.W., Li, H, Structure of an ancient respiratory system. Cell – Revised Manuscript Submitted

IN PREPARATION

Tokmina-Lukaszewska, M, Berry, L, Patterson, A, Scott, L, Balasubramanian, N, Bothner, B. Mass spectrometry-based structure-function analysis of bifurcating enzyme complexes. Frontiers in Microbiology. In preparation.

Artz, J.H., Mulder, D.W., Tokmina-Lukaszewska, M., Zadvornyy, O.A, Pence, N., Bothner, B., Raugei, S., Peters, J.W., King, P.W. The catalytic mechanism of Clostridium pasteurianum [FeFe]-hydrogenase II proceeds through a destabilized 1-electron state. JACS - in preparation.

Zhai, Y., Seefeldt, L.C., Raugei, S., Beratan, D.N. Pathway switching in nitrogenase induced by ATP binding. In preparation.

Yuly, J., Zhang, P., Lubner, C., Beratan, D.N. On the time scale of charge flow through bifurcating enzymes. In preparation.

Zadvornyy, O.A, Keable, S.M., Cohen, A.E., Johnson, L., Ginovska, B., Artz, J.H., Song, J., McPhillips, S.E., Lyubimov, A.Y., Davidson, C.M,, Soltis, S.M., Raugei, S., Peters, J.W. Dynamic secondary interactions in tuning the reactivity of an [FeFe]-hydrogenase. In preparation.

Williams, S.G., Artz, J.H., Mulder, D.W., Ratzloff, M.W., King, P.W., Peters, J.W., Jones, A. K. Electrochemical definition of the unique catalytic profile of [FeFe]-hydrogenase II from Clostridium pasteurianum. Chem Commun – in preparation to submit before 9 March.

Jennings, D.P., Schut, G.J., Nguyen, D.M.N, Adams, M.W.W., Jones, A.K. The electron-bifurcating NADH-dependent reduced ferredoxin: NADP+ oxidoreductase is tuned for NADP+ reduction. J Biol Chem - in preparation.

Lubner, C.E., Mulder, D.W., Ward, R., Artz, J., Peters, J.W., Smalyukh, I., King, P.W. Functional insights into the electronic and magnetic properties of the non-canonical distal iron-sulfur cluster of [FeFe]-hydrogenase. J Am Chem Soc - in preparation.

Poudel, S., Boyd, E.S., Mapping the genomic landscape of electron bifurcation. Front Microbio - in preparation.

Berry, L., Tokmina-Lukaszewska, M., Harris, D.F., Zadvornyy, O.A., Raugei, S., Peters, J.W., Seefeldt, L.C., Bothner B., Development and characterization of a structural model of the Fe-nitrogenase. Biochim Biophys Acta - in preparation.

Seefeldt, L.C., Hoffman, B.M., Peters, J.W., Dean, D.R., Raugei, S., Beratan, D.N. Energy Transduction in Nitrogenase. Acc Chem Res - Invited review in preparation.

Schut, G., Raseek, M., Nguyen, D.M.N., Lipscomb, G., Mulder, D., Tokmina-Lukaszewska, M., Hoben, J., Lubner, C., King, P.W., Peters, J.W., Bothner, B., Miller, A.F., Adams, M.W.W. The bifurcating electron transfer flavoprotein (EtfABCX) complex of Pyrobaculum aerophilum displays a distinct charge transfer complex with NADH. J Biol Chem - In preparation.